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mem non-essential amino acids solution (mem-neaa), 100  (Thermo Fisher)


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    Thermo Fisher mem non-essential amino acids solution (mem-neaa), 100
    Mem Non Essential Amino Acids Solution (Mem Neaa), 100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mem+non-essential+amino+acids+solution+(100/mem+non+essential+amino+acids/pm39915620-419-57-65
    Average 90 stars, based on 1 article reviews
    mem non-essential amino acids solution (mem-neaa), 100 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Cytotoxicity Assay:

    Article Title: Directed differentiation of neural-stem cells and subtype-specific neurons from hESCs
    Article Snippet: MEM non-essential amino acids solution 100× (Invitrogen, cat. no. 11140): ready to use.

    Article Title: Engineering a CRISPRoff Platform to Modulate Expression of Myeloid Cell Leukemia (MCL-1) in Committed Oligodendrocyte Neural Precursor Cells
    Article Snippet: MEM non-essential amino acids solution 100× (Thermo Fisher Scientific, catalog number: 11140050) 60.

    Article Title: Viridistratins A−C, Antimicrobial and Cytotoxic Benzo[ j ]fluoranthenes from Stromata of Annulohypoxylon viridistratum (Hypoxylaceae, Ascomycota)
    Article Snippet: Table S2: Cytotoxicity assay experiment parameters cell line type No. growth medium L929 mouse fibroblasts ACC 2 DMEM 1 + 10 % FBS 2 KB 3.1 human endocervical adenocarcinoma (AC) ACC 158 DMEM 1 + 10 % FBS 2 PC-3 human prostate AC ACC 465 F-12K Nutmix 3 + 10 % FBS 2 SK-OV-3 human ovary AC n/a McCoys 5a 4 + 10 % FBS 2 MCF-7 human breast AC ACC 115 RPMI 1640 5 + 10 % FBS 2 + 1 % MEMNEAA 6 + 1.25 mL/500 mL insulin 7 A431 human squamous AC ACC 91 RPMI 1640 4 + 10 % FBS 2 A549 human lung carcinoma ACC 107 DMEM 1 1 DMEM: Dulbecco’s Modified Eagle Medium (SN 61965026, Thermo Fisher Scientific, Waltham, MA, USA) 2 FBS: Fetal Bovine Serum (SN 10500064, Thermo Fisher Scientific) 3 F-12K Nutmix: Ham’s F-12K (Kaign’s) Medium (SN 21127022, Thermo Fisher Scientific) 4 McCoys 5a: McCoy’s 5a (modified) Medium (SN 26600023, Thermo Fisher Scientific) 5 RPMI 1640: RPMI 1640 Medium (SN 21875091, Thermo Fisher Scientific) 6 MEMNEAA: MEM Non-Essential Amino Acids Solution 100× (SN 11140035, Thermo Fisher Scientific) 7 Insulin: Human Recombinant Insulin, Zinc Solution (SN 12585014, Thermo Fisher Scientific) [S2] Sandargo, B.; Michehl, M.; Praditya, D.; Steinmann, E.; Stadler, M.; Surup, F. Antiviral Meroterpenoid Rhodatin and Sesquiterpenoids Rhodocoranes A-E from the Wrinkled Peach Mushroom, Rhodotus palmatus.

    Article Title: The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei
    Article Snippet: Here, 1 l DTM medium contains 6.8 g NaCl, 0.4 g KCl, 0.2 g CaCl 2 , 0.14 g NaH 2 PO 4 , 0.2 g MgSO 4 , 7.94 g HEPES, 2.2 g NaHCO 3 , 1 ml phenol red (10 mg/ml), 20 ml MEM amino acids solution 50× (Thermo Fisher, ref no. 11130036), 10 ml MEM non-essential amino acids solution 100× (Thermo Fisher, ref no. 11140035), 1.63 g l -glutamine, 0.293 g l -glutamic acid hydrochloride, 730 μl glycerol, 10 ml MEM vitamin solution 100× (Thermo Fisher, ref no. 11120037), 14 μl β -mercaptoethanol, 114 mg Na-pyruvate, 14 mg hypoxanthine, 28.2 mg bathocuproin, 7.5 mg hemin, 640 mg proline, and 182 mg cysteine.

    Modification:

    Article Title: Directed differentiation of neural-stem cells and subtype-specific neurons from hESCs
    Article Snippet: MEM non-essential amino acids solution 100× (Invitrogen, cat. no. 11140): ready to use.

    Article Title: Engineering a CRISPRoff Platform to Modulate Expression of Myeloid Cell Leukemia (MCL-1) in Committed Oligodendrocyte Neural Precursor Cells
    Article Snippet: MEM non-essential amino acids solution 100× (Thermo Fisher Scientific, catalog number: 11140050) 60.

    Article Title: Viridistratins A−C, Antimicrobial and Cytotoxic Benzo[ j ]fluoranthenes from Stromata of Annulohypoxylon viridistratum (Hypoxylaceae, Ascomycota)
    Article Snippet: Table S2: Cytotoxicity assay experiment parameters cell line type No. growth medium L929 mouse fibroblasts ACC 2 DMEM 1 + 10 % FBS 2 KB 3.1 human endocervical adenocarcinoma (AC) ACC 158 DMEM 1 + 10 % FBS 2 PC-3 human prostate AC ACC 465 F-12K Nutmix 3 + 10 % FBS 2 SK-OV-3 human ovary AC n/a McCoys 5a 4 + 10 % FBS 2 MCF-7 human breast AC ACC 115 RPMI 1640 5 + 10 % FBS 2 + 1 % MEMNEAA 6 + 1.25 mL/500 mL insulin 7 A431 human squamous AC ACC 91 RPMI 1640 4 + 10 % FBS 2 A549 human lung carcinoma ACC 107 DMEM 1 1 DMEM: Dulbecco’s Modified Eagle Medium (SN 61965026, Thermo Fisher Scientific, Waltham, MA, USA) 2 FBS: Fetal Bovine Serum (SN 10500064, Thermo Fisher Scientific) 3 F-12K Nutmix: Ham’s F-12K (Kaign’s) Medium (SN 21127022, Thermo Fisher Scientific) 4 McCoys 5a: McCoy’s 5a (modified) Medium (SN 26600023, Thermo Fisher Scientific) 5 RPMI 1640: RPMI 1640 Medium (SN 21875091, Thermo Fisher Scientific) 6 MEMNEAA: MEM Non-Essential Amino Acids Solution 100× (SN 11140035, Thermo Fisher Scientific) 7 Insulin: Human Recombinant Insulin, Zinc Solution (SN 12585014, Thermo Fisher Scientific) [S2] Sandargo, B.; Michehl, M.; Praditya, D.; Steinmann, E.; Stadler, M.; Surup, F. Antiviral Meroterpenoid Rhodatin and Sesquiterpenoids Rhodocoranes A-E from the Wrinkled Peach Mushroom, Rhodotus palmatus.

    Article Title: The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei
    Article Snippet: Here, 1 l DTM medium contains 6.8 g NaCl, 0.4 g KCl, 0.2 g CaCl 2 , 0.14 g NaH 2 PO 4 , 0.2 g MgSO 4 , 7.94 g HEPES, 2.2 g NaHCO 3 , 1 ml phenol red (10 mg/ml), 20 ml MEM amino acids solution 50× (Thermo Fisher, ref no. 11130036), 10 ml MEM non-essential amino acids solution 100× (Thermo Fisher, ref no. 11140035), 1.63 g l -glutamine, 0.293 g l -glutamic acid hydrochloride, 730 μl glycerol, 10 ml MEM vitamin solution 100× (Thermo Fisher, ref no. 11120037), 14 μl β -mercaptoethanol, 114 mg Na-pyruvate, 14 mg hypoxanthine, 28.2 mg bathocuproin, 7.5 mg hemin, 640 mg proline, and 182 mg cysteine.

    Recombinant:

    Article Title: Directed differentiation of neural-stem cells and subtype-specific neurons from hESCs
    Article Snippet: MEM non-essential amino acids solution 100× (Invitrogen, cat. no. 11140): ready to use.

    Article Title: Engineering a CRISPRoff Platform to Modulate Expression of Myeloid Cell Leukemia (MCL-1) in Committed Oligodendrocyte Neural Precursor Cells
    Article Snippet: MEM non-essential amino acids solution 100× (Thermo Fisher Scientific, catalog number: 11140050) 60.

    Article Title: Viridistratins A−C, Antimicrobial and Cytotoxic Benzo[ j ]fluoranthenes from Stromata of Annulohypoxylon viridistratum (Hypoxylaceae, Ascomycota)
    Article Snippet: Table S2: Cytotoxicity assay experiment parameters cell line type No. growth medium L929 mouse fibroblasts ACC 2 DMEM 1 + 10 % FBS 2 KB 3.1 human endocervical adenocarcinoma (AC) ACC 158 DMEM 1 + 10 % FBS 2 PC-3 human prostate AC ACC 465 F-12K Nutmix 3 + 10 % FBS 2 SK-OV-3 human ovary AC n/a McCoys 5a 4 + 10 % FBS 2 MCF-7 human breast AC ACC 115 RPMI 1640 5 + 10 % FBS 2 + 1 % MEMNEAA 6 + 1.25 mL/500 mL insulin 7 A431 human squamous AC ACC 91 RPMI 1640 4 + 10 % FBS 2 A549 human lung carcinoma ACC 107 DMEM 1 1 DMEM: Dulbecco’s Modified Eagle Medium (SN 61965026, Thermo Fisher Scientific, Waltham, MA, USA) 2 FBS: Fetal Bovine Serum (SN 10500064, Thermo Fisher Scientific) 3 F-12K Nutmix: Ham’s F-12K (Kaign’s) Medium (SN 21127022, Thermo Fisher Scientific) 4 McCoys 5a: McCoy’s 5a (modified) Medium (SN 26600023, Thermo Fisher Scientific) 5 RPMI 1640: RPMI 1640 Medium (SN 21875091, Thermo Fisher Scientific) 6 MEMNEAA: MEM Non-Essential Amino Acids Solution 100× (SN 11140035, Thermo Fisher Scientific) 7 Insulin: Human Recombinant Insulin, Zinc Solution (SN 12585014, Thermo Fisher Scientific) [S2] Sandargo, B.; Michehl, M.; Praditya, D.; Steinmann, E.; Stadler, M.; Surup, F. Antiviral Meroterpenoid Rhodatin and Sesquiterpenoids Rhodocoranes A-E from the Wrinkled Peach Mushroom, Rhodotus palmatus.

    Article Title: The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei
    Article Snippet: Here, 1 l DTM medium contains 6.8 g NaCl, 0.4 g KCl, 0.2 g CaCl 2 , 0.14 g NaH 2 PO 4 , 0.2 g MgSO 4 , 7.94 g HEPES, 2.2 g NaHCO 3 , 1 ml phenol red (10 mg/ml), 20 ml MEM amino acids solution 50× (Thermo Fisher, ref no. 11130036), 10 ml MEM non-essential amino acids solution 100× (Thermo Fisher, ref no. 11140035), 1.63 g l -glutamine, 0.293 g l -glutamic acid hydrochloride, 730 μl glycerol, 10 ml MEM vitamin solution 100× (Thermo Fisher, ref no. 11120037), 14 μl β -mercaptoethanol, 114 mg Na-pyruvate, 14 mg hypoxanthine, 28.2 mg bathocuproin, 7.5 mg hemin, 640 mg proline, and 182 mg cysteine.



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    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
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    Thermo Fisher mem non-essential amino acids (100 × solution; neaa
    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
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    Image Search Results


    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy medium (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.

    Journal: Bio-protocol

    Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)

    doi: 10.21769/BioProtoc.5304

    Figure Lengend Snippet: (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy medium (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.

    Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

    Techniques: Derivative Assay, Control, Staining, Marker, Expressing